- 生物活性
NSC 66811 是一个MDM2-p53 抑制剂，其结合到MDM2 的 Ki 值为 120 nM。
- 体外研究
NSC 66811 (0, 5, 10, 20 μM) dose-dependently induces the accumulation of p53, MDM2, and p21
cip1/waf
due to the functional activation of p53.
Western Blot Analysis
Cell Line:
HCT-116 (p53
+/+
and p53
-/-
) cells
[1]
.
Concentration:
0, 5, 10, 20 μM.
Incubation Time:
48 h.
Result:
Dose-dependently induced the accumulation of p53, MDM2, and p21
cip1/waf
proteins in the HCT-116 human colon cancer cell line with wild-type p53.
Had no effect on the levels for p53, MDM2, and p21
cip1/waf
protein in the isogenic HCT-116 p53
-/-
cell line.
