- 英文名称
- Hexachloro-6-carboxyfluorescein
- 分子式
- C21H6Cl6O7
- 分子量
- 582.99
- 中文别名
- 6-羧基-2',4,4',5',7,7'-六氯荧光素; 5/6-羧基六氯荧光素; 6-羧基六氯荧光素; 2',4,4',5',7,7'-六氯-3',6'-二羟基-3-氧代-3H-螺[异苯并呋喃-1,9'-氧杂蒽]-6-羧酸; 6-HEX; 5(6)-HEX; 2',4,4',5',7,7'-Hexachloro-3',6'-dihydroxy-3-oxo-3H-spiro-[isobenzofuran-1,9'-xanthene]-6-carboxylic acid; 2',4,4',5',7,7'-Hexachloro-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-6-carboxylic acid; 2',4,5',7'-Tetrachloro-6-carboxy-4,7-dichlorofluorescein; 2',4,4',5',7,7'-Hexachloro-3',6'-dihydroxy-3-oxo-3H-spiro-[isobenzofuran-1,9'-xanthene]-6-carboxy; 2',4,5′,7′-tetrachloro-5(6)-carboxy-4,7-dichlorofluorescein; 2',4,4',5',7,7'-hexachloro-3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylic acid
- 用途
- 六氯-6-羧基荧光素是荧光基团，激发波长532 nm，发射波长556 nm；可用于标记核酸序列，设计荧光引物；标记的核酸可用氩激光543 nm射线识别，在550和650 nm发射光谱（5 nm带宽）下实现复杂组织或异质细胞群中基因定位。
