- 英文名称
- 5-Fluoroorotic acid
- 分子式
- C5H3FN2O4
- 分子量
- 174.09
- 中文别名
- 5-氟-2,6-二氧代-1,2,3,6-四氢嘧啶-4-羧酸; 5-氟乳清酸水合物; 5-氟乳清酸一水化合物; 5-氟乳清酸一水物; 1,2,3,6-tetrahydro-2,6-dioxo-5-fluoro-4-pyrimidinecarboxylicaci; 1,2,3,6-tetrahydro-2,6-dioxo-5-fluoro-4-pyrimidinecarboxylicacid; 5-fluoro-2,4-dioxo-1H-pyrimidine-6-carboxylic acid hydrate; nsc31712; ro2-9945; 2,6-Dihydroxy-5-fluoropyrimidine-4-carboxylic acid; 5-FLUOROURACIL-4-CARBOXYLIC ACID; 5' FOA; 4-Pyrimidinecarboxylic acid, 5-fluoro-1,2,3,6-tetrahydro-2,6-dioxo-; 5-Fluoro-6-carboxyuracil; 5-Fluoro orotic acid; 5-Fluoro-2,6-dioxo-1,2,3,6-tetrahydropyrimidine-4-carboxylic acid; 5-Fluoro-2,6-dioxo-1,2,3,6-tetrahydro-4-pyrimidinecarboxylic acid; 5-Fluoroorotic acid monohydrate
- 用途
- 1. 酵母反选：用于酵母菌质粒清除、质粒穿梭、等位置换和双杂交筛选，利用URA3基因编码的脱羧酶将其转化为有毒的5-氟尿嘧啶实现筛选；2. 检测酵母URA3基因表达；3. 构建酵母双杂交文库；4. 其他酵母遗传学领域（基因替换、可移动遗传因子分析、基因突变鉴定）；5. 作为B淋巴母细胞系（如FO细胞系）培养的试剂。
