- 生物活性
5β-Dihydrocortisol，氢化可的松 (Cortisol) 的代谢产物，是潜在的盐皮质激素。5β-Dihydrocortisol 可以增强糖皮质激素的活性以提高眼压。5β-Dihydrocortisol 可引起乳腺癌细胞凋亡。
- 体外研究
5β-Dihydrocortisol (10-100 μM; 48 h) inhibits the viability of MCF-7 cells, with an IC
50
of 27.59 μM.
5β-Dihydrocortisol (14 μM; 24 h) induces 35.6% early apoptosis and 2.5% late apoptosis in MCF-7 cells.
5β-Dihydrocortisol (1-10 μM; 48 h) quenches the intrinsic fluorescence of human serum albumin (HAS) with a maximum emission peak at 360 nm, with no shift in fluorescence peak.
Cell Viability Assay
Cell Line:
MCF-7 and HEK 293 cells
Concentration:
10, 20, 40, 60, 80, 100 μM
Incubation Time:
48 hours
Result:
Inhibited the viability of MCF-7 cells in a dose-dependent manner.
No toxicity in terms of cell viability was observed with HEK293 cell line.
Apoptosis Analysis
Cell Line:
MCF-7 cells
Concentration:
14 μM
Incubation Time:
24 hours
Result:
Induced 35.6% and 2.5% of early and late apoptosis.
- 体内研究
5β-Dihydrocortisol (0.1-1.0%; 18 d) potentiates the action of topically applied Dexamethasone (0.06%) in raising the intraocular pressure (IOP) in young rabbits.
