1482-50-4 11β,17α,21-三羟基-5β-孕甾烷-3,20-二酮

• 生物活性 5β-Dihydrocortisol，氢化可的松 (Cortisol) 的代谢产物，是潜在的盐皮质激素。5β-Dihydrocortisol 可以增强糖皮质激素的活性以提高眼压。5β-Dihydrocortisol 可引起乳腺癌细胞凋亡。
• 体外研究 5β-Dihydrocortisol (10-100 μM; 48 h) inhibits the viability of MCF-7 cells, with an IC 50 of 27.59 μM. 5β-Dihydrocortisol (14 μM; 24 h) induces 35.6% early apoptosis and 2.5% late apoptosis in MCF-7 cells. 5β-Dihydrocortisol (1-10 μM; 48 h) quenches the intrinsic fluorescence of human serum albumin (HAS) with a maximum emission peak at 360 nm, with no shift in fluorescence peak. Cell Viability Assay Cell Line: MCF-7 and HEK 293 cells Concentration: 10, 20, 40, 60, 80, 100 μM Incubation Time: 48 hours Result: Inhibited the viability of MCF-7 cells in a dose-dependent manner. No toxicity in terms of cell viability was observed with HEK293 cell line. Apoptosis Analysis Cell Line: MCF-7 cells Concentration: 14 μM Incubation Time: 24 hours Result: Induced 35.6% and 2.5% of early and late apoptosis.
• 体内研究 5β-Dihydrocortisol (0.1-1.0%; 18 d) potentiates the action of topically applied Dexamethasone (0.06%) in raising the intraocular pressure (IOP) in young rabbits.

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