- 生物活性
TNP-351 是一种抗叶酸剂。TNP-351 是一种二氢叶酸还原酶 (DHFR) 抑制剂，在体内外对白血病和实体瘤细胞均具有有效的抗肿瘤活性。
- 靶点
Antifolate, DHFR
- 体外研究
TNP-351 inhibits the proliferation of mouse L1210 leukemia cells and human CCRF-CEM lymphoblastic leukemia cells with ED
50
values of 0.79 and 2.7 nM, respectively.
The ED
50
values determined for the parent cell line CCRF-CEM, CCRFCEM R30/6 subline, CCRF-CEM R1, and CCRF-CEM R2 are 2.7, 5.8, 94 and 76 nM, respectively.
Cell Viability Assay
Cell Line:
L1210 and CCRF-CEM cells
Concentration:
0.1, 0.3, 1, 3, and 10 nM for L1210 cells; 0.3, 1, 3, 10, and 30 for CCRF-CEM cells
Incubation Time:
48 hours for L1210 cells; 72 hours for CCRF-CEM cells
Result:
The ED
50
values were 0.79 and 2.7 nM for L1210 and CCRF-CEM cells, respectively.
