体外研究
Suberoyl bis-hydroxamic acid (10, 20 or 50 μM; 24 hours) combination with TRAIL improves apoptosis extent, and when TRAIL is combined with 20 μM SBHA (itself causing only 10–15% apoptosis), resulting in 45–50% cell death.Suberoyl bis-hydroxamic acid (20-50 μM; 10-20 hours) alone has little effect on the expression of the proteins Bcl-xL, Mcl-1, and has albeit mildeffect on Bax. When it combines with TRAIL,which increases the ratio of relative protein expression of Bcl-xL and Bax in early periods, while the change in the ratio of Mcl-1 and Bax increases later in MM-BI and Ist-Mes2 cells.Suberoyl bis-hydroxamic acid (30 μM; 6 hours) causes accumulation of acetylated histone H4 in MEL cells.
Apoptosis Analysis
Cell Line:
MM-BI and Ist-Mes2 cells
Concentration:
10 μM, 20 μM or 50 μM
Incubation Time:
24 hours
Result:
Showed a cooperative effect in cell apoptosis.
体内研究
Suberoyl bis-hydroxamic acid (intraperitoneal injection; 200 mg/kg; every 2 days; 12 days) reveals a marked increase in the active form of Notch1 (NICD) with a concomitant decrease in ASCL1. It reduces the MTC tumor growth.
Animal Model:
Nude mice injected with human MTC cells
Dosage:
200 mg/kg
Administration:
Intraperitoneal injection; every 2 days; 12 days
Result:
Resulted in an average 55% inhibition of tumor growth in SBHA treatment group.
