Analysis Note Protein determined by biuret.Unit Definition One unit will hydrolyze 1.0 μmole of N-acetyl-L-methionine per hr at pH 7.0 at 25 °C.Biochem/physiol Actions The enzyme hydrolyzes a variety of N-acyl-L-amino acids and dehydropeptides containing dehydroalanyl residues. N-acyl-D-amino acids and dehydropeptides other than dehydroalanine are not hydrolyzed. The following compounds are hydrolyzed by acylase I: chloroacetyl-L-amino acids, L-leucinamide, chloroacetyldehydroalanine, glycyl-L-amino acids, and glycyldehydroalanine. Aminoacylase is widely used as a reagent to resolve amino acid racemates. The Km for acyl amino acids have been reported in the literature: Ac-L-Met (0.99 mM), Ac-L-Glu (10.2 mM), and Ac-L-Phe (5.5 mM).Aminoacylase from pig kidney is activated by Zn2+ and Co2+ and inhibited by SH blocking reagents, disulfide reducing reagents, N-tosyl-L-alanine,DL-norleucine, and N-tosyl-L-lysine chloromethylketone.
