Procion Blue HB; CIBRACONBLUEF3GA; Kayacion Blue A-B; C.I.REACTIVEBLUE2; Reactive blue 2 (C.I. 61211); 1-Amino-4-[[4-[[4-chloro-6-[[4-(sodiooxysulfonyl)phenyl]amino]-1,3,5-triazin-2-yl]amino]-3-(sodiooxysulfonyl)phenyl]amino]-9,10-dihydro-9,10-dioxo-2-anthracenesulfonic acid sodium salt
物化属性
储存条件
2-8°C
外观
深蓝，生理盐水悬浮液
溶解性
可溶于水
稳定性
稳定，与强氧化剂不相容
安全信息
安全说明
WGK Germany:3
F:10-21
TSCA:TSCA listed
生产及用途
用途与制备
生物活性
Procion Blue HB (Reactive Blue 2)是嘌呤能拮抗剂。
体外研究
Reactive Blue 2 is used as an ATP receptor antagonist and induces Ca
2+
oscillations in HeLa cells. Reactive Blue 2 enhances a Ca
2+
response to histamine that is linked to the PLC cascade. Reactive Blue 2 may activate the PLC cascade in an extracellular Ca
2+
-dependent manner and induce Ca
2+
oscillations. The application of Reactive Blue 2 increases K
+
secretion in a dose-dependent manner, and this increase is characterized as a peak followed by a partial relaxation to a steady-state. Reactive Blue 2 has antagonistic activities at P2Y4, and the antagonist potency at P2Y4 paralleled the potency of K+ secretion.The anthraquinone dye reactive blue 2 is found to be a potent inhibitor of a protein kinase isolated and purified from thylakoids. The mode of inhibition is noncompetitive, with a K
i
of 8 μM for the membrane-bound kinase, and 6 microM for the purified kinase. The inhibitor does not modify the substrate preference of the endogenous kinase and could be removed from the membrane by washing. Reactive blue 2 selectively inhibits responses mediated via the P2ypurinoceptor, at least within a limited concentration range. In preparations where the tone has been raised with noradrenaline, ATP and 2-methylthio ATP, but not α,β-methylene ATP, produce relaxations of the vessel. These relaxations are inhibited in the presence of reactive blue 2. Reactive blue 2, at concentrations of 0.3-10 μM blocks the ATP-induced oscillation in a concentration-dependent manner.
