3316-02-7 8-羟基-1,3,6-萘三磺酸

TSCA:TSCA listed

• 生物活性 ζ-Stat (NSC37044) 是一种特异且非典型的 PKC-ζ 的抑制剂，IC50 值为 5 μM。ζ-Stat 可以减少黑色素瘤细胞系的增殖并诱导细胞凋亡，在体外具有抗肿瘤活性。
• 靶点 aPKC-ζ 5 μM (IC 50 )
• 体外研究 ζ-Stat (0.1-20 µM) shows only 13% inhibition on PKC-ι at 20 μM, but shows a significant inhibition on PKC-ζ as 51% at 5 μM level. ζ-Stat (0.1-10 µM; 3 d) significantly decreases cell proliferation of SK-MEL-2 and MeWo upon increasing the concentrations. ζ-Stat (7 or 10 µM; 24-72 h) and 5-FU in combination is able to decrease the viability of LoVo CRC cells by more than 75%. ζ-Stat (5 µM; 3 d) shows a significant diminution of phosphorylated, total PKC-ζ, Bcl-2 and PARP levels, and increases Caspase-3 and cleaved-PARP levels in SK-MEL-2 and MeWo cells. ζ-Stat (5 µM; 1-10 h) does not show significant cytotoxicity on MEL-F-NEO, SK-MEL-2 and MeWo cells. Cell Proliferation Assay Cell Line: MEL-F-NEO, SK-MEL-2 and MeWo cells Concentration: 0.1, 0.5, 1, 2.5, 5, 7.5, 10 µM Incubation Time: 3 days Result: Decreased proliferation by 47.7% for 5 µM in SK-MEL-2 cells and by 50.6% for 5 µM in MeWo cells. Showed significant inhibitions on MEL-F-NEO cells 19.3% (P ≤ 0.05) at 10 μM. Western Blot Analysis Cell Line: SK-MEL-2 and MeWo cells Concentration: 5 µM Incubation Time: 3 days Result: Decreased phosphorylated and total PKC-ζ levels.

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