化学合成。
化学合成
合成路线 1（1. 合成：123148-78-7）
产率：100%
合成条件：With N-iodo-succinimide; sodium sulfate In ethanol; N,N-dimethyl-formamide
实验步骤：步骤1. 4-氯-5-碘-7H-吡咯并[2,3-d]嘧啶（化合物3）4-氯-7H-吡咯并[2,3-d]嘧啶10.75g（70mmol）（Toronto Research 将化学品（Inc）和N-碘代琥珀酰亚胺（16.8g，75mmol）溶解在400mL无水DMF中，并在环境温度下在黑暗中放置过夜。 蒸发溶剂。 将黄色残余物悬浮在热的10％Na 2 SO 3溶液中，过滤，用热水洗涤两次并从乙醇中结晶，得到14.6g（74.6％）标题化合物，为灰白色晶体。 将母液蒸发至1/3体积并再次从乙醇中结晶，得到2.47g（12.3％）标题产物。 总收益率接近100％。
参考文献：
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合成路线 2（2. 合成：123148-78-7）
产率：86.9%
合成条件：at 20℃;
实验步骤：步骤1：将4-氯-7H-吡咯并[2,3-d]嘧啶10.75g（70mmol）和N-碘代琥珀酰亚胺（16.8g，75mmol）溶于400mL无水DMF中，并在环境温度下保持在室温下。 黑夜过夜。 蒸发溶剂。 将黄色残余物悬浮在热的10％Na 2 SO 3溶液中，过滤，用热水洗涤两次并从乙醇中结晶，得到14.6g（74.6％）标题化合物，为灰白色晶体。 将母液蒸发至体积并再次从乙醇中结晶，得到2.47g（12.3％）目标化合物。 总产率接近100％; Tm 212-214℃（dec）;UVλmax：307,266,230,227nm（甲醇）; MS：277.93（MH），313（M + Cl）; 1H- NMR（DMSO-d6）：12.94（s，1H，NH），8.58（s，1H），7.94（s，1H）。
参考文献：
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